https://digitallibrary.bldedu.ac.in/xmlui/handle/123456789/114 2026-04-23T13:53:52Z https://digitallibrary.bldedu.ac.in/xmlui/handle/123456789/4628 A Comparative Study Of Cartridge Based Nucleic Acid Amplification Testing And Auramine-Rhodamine Stain Microscopy With Culture Method In The Diagnosis Of Pulmonary Tuberculosis Dr.Hajra Tasneem Masali One of the crucial step for effective tuberculosis management is to choose a Diagnostic method with high sensitivity and specificity. But slow growth rate of Tubercle bacilli makes the diagnosis more complicated and hence need of hour is improved diagnostic methods to limit progression and the spread of the disease. Options available among conventional laboratory methods are sputum microscopy and culture methods. Sputum microscopy is rapid, reliable, simple & reasonably costing but has poor sensitivity and requires multiple visits leading to higher default rate. Culture method although considered gold standard but it takes almost 2-6 weeks’ time to give the result and also requires proper infrastructure with technical expertise. But with advancement in technology CBNAAT also offer a better option as it is specific, automated technology utilizing DNA - PCR technique for identification. It is currently unique in its simplification of molecular testing. It can detect both M. tuberculosis and Rifampicin resistance directly from sputum in an assay fetching results within two hours 2019-01-01T00:00:00Z https://digitallibrary.bldedu.ac.in/xmlui/handle/123456789/2446 Comparison Of Lowenstein-Jensen Medium, Middlebrook 7h10 Medium And Bact/Alert 3d For Isolation Of Mycobacterium Tuberculosis From Clinical Specimens Naveen.G Introduction – Tuberculosis is the most common cause of death due to single infectious agent worldwide in adults. India alone accounts for 30% of global tuberculosis burden. There is manifest need for method of cultivation of mycobacteria that is reliable, economical and has short turnaround time. Objective – Present study was attempted to assess the feasibility of using MB bact and middlebrook7H10 as primary isolation medium for mycobacteria. It has been compared with LJ medium, the gold standard. Materials and Methods – Various clinical specimens from total of 230 clinically suspected cases of TB were studied. All isolates were decontaminated using modified Petroff’s method. Each sample was subjected to ZN staining and simultaneously inoculated onto LJ medium, middlebrook7H10 medium and MB bact. Growth from the culture were confirmed by ZN staining and speciated using biochemical reactions. Results – Out of 230 samples screened, 116 isolates were obtained. Of the 116 all of them were isolated from MB bact, 82 were isolated by LJ medium and 62 were isolated by middlebrook7H10. 82 isolates were obtained by MB bact and LJ medium, 62 were obtained by middlebrook7H10 and MB bact, 58 by LJ and middlebrook7H10 and 58 by LJ medium, middlebrook7H10 and MB bact. Neither L J medium nor middlebrook 7H 1O medium could isolate mycobacteria exclusively. The average isolation time by L J, middlebrook 7H10 medium and MB BACT was 30.81 days,31.06 days and 18.70 days. 2012-01-01T00:00:00Z https://digitallibrary.bldedu.ac.in/xmlui/handle/123456789/891 Serological Markers Hbsag And Hbeag In Chronic Hepatitis B Carriers And Their Correlation With Viral Load By Polymerase Chain Reaction Assay Borgaonkar, Rasika 2018-01-01T00:00:00Z https://digitallibrary.bldedu.ac.in/xmlui/handle/123456789/592 Seroprevalence of Rubella antibodies in women of reproductive age Shilpi, Gupta 2015-01-01T00:00:00Z