Effect of metal ions for the biosynthesis of fibrinolytic enzyme from aspergillus tamari.

Abstract

Thrombolytic agents are used to treat heart attack, stroke, deep vein thrombosis, pulmonary embolism and occlusion of a peripheral artery or in dwelling catheter1. All thrombolytic agents are serine proteases and convert plasminogen to plasmin (EC 3.4.21.7), which breaks down the fibrinogen and fibrin and dissolves the clot. Fibrinolytic enzyme production was investigated in the filamentous fungi Aspergillus tamarii SAS 02 a soil isolate from different regions of Karnataka and produces 152 IU. The process economization were employed to achieve higher yield of fibrinolytic enzyme through submerged fermentation (SmF) and here we made an attempt to incorporate metal ions as a trace elements sources such as copper sulphate (CuSO4), iron sulphate (FeSO4), Zinc sulphate (ZnSO4) and Magnesium sulphate (MgSO4) source to the production medium. In case of zinc sulphate the maximum fibrinolytic enzyme production of 152 IU was observed at 0.02% and where as copper sulphate (CuSO4), iron sulphate (FeSO4) and magnesium sulphate (MgSO4) yielded maximum fibrinolytic enzyme of 71 IU, 98 IU and 95 IU