"Multiple drug resistance (MDR) and phenotypic detection of extended spectrum β-lactamases (ESBL) and metallo β-lactamases (MBL) producing enterobacteriaceae isolates of neonatal sepsis."

Abstract

Neonatal sepsis is one of the major causes of morbidity and mortality in newborns. In developing countries, multiple drug resistant (MDR) organisms causing neonatal sepsis are threat to current b lactam therapy leading to treatment failure. Emergence of ESBLs and MBLs are a vital factor in the treatment of infections associated with sepsis. This study aims at phenotypic detection of Extended spectrum β-lactamases (ESBL) and Metallo β-lactamases (MBL) producing Enterobacteriaceae isolates of Neonatal Sepsis and to assess the burden of MDR. Total 19 clinical isolates of Enterobacteriaceae were isolated from 115blood samples of suspected cases. Antimicrobial susceptibility was determined by Kirby–Bauer's disk diffusion method. Isolates were screened for ESBL and MBL production by Clinical and Laboratory Standards Institute (CLSI) disk method, and confirmation was done by CLSI phenotypic disk confirmatory test.