New Methylene Blue Stain for Malaria Detection on Thin Smears.

Abstract

Malaria is the most important parasitic infection of man. Microscopy remains gold standard in malaria diagnosis. Management of malaria requires rapid detection of parasite in human blood. Hence there is a need to develop another diagnostic method with less limitation, which will address this issue. Aim and Objectives: To find a low cost reliable and accurate method for malaria detection on peripheral smear. Material and Methods: A prospective study of 40 cases was done. Two thin smears were prepared for each case; one was stained with Leishman stain and other with new methylene blue stain and examined under oil immersion. The smears were examined individually by two pathologists and results were prepared.