"Isolation, Identification and Antimicrobial Susceptibility of Brucella Isolates From Human Cases."

Abstract

Introduction: Brucellosis is a worldwide zoonotic disease that remains an important public health problem in India. As clinical manifestations of human brucellosis are variable in nature, and no constellation of clinical findings can be considered characteristic laboratory help is must in the diagnosis. In the laboratory, brucellosis is generally diagnosed by serological tests. Though many serological tests with different principles are available, serological testing does not provide direct evidence for the presence of the pathogen, hence isolation of Brucella spp. from the clinical specimen is considered to be the gold standard. Brucella is highly infectious and requires level 3 bio-containment facilities and technically skilled personnel, Brucella cultures are rarely performed. Due to the rampant use of rifampin for the treatment of tuberculosis and reports on development of resistance in virtually all organisms the sensitivity of Brucellae to the traditional drugs cannot be presumed.