Abstract:
BACKGROUND
Cytological study of body fluid is a complete diagnostic modality.The accurate
identification of cells as either malignant or reactive mesothelial cells is a diagnostic
problem in conventional cytological smears.Distinguishing benign from malignant
cellular changes requires meticulous screening and understanding of the range of reactive
changes.
Routine centrifuge is not satisfactory in reporting fluids with scant cellularity.
Hence for fluids with scant cellularity, cytocentrifuge and cell block are useful methods.
Also the morphology of the cells is well appreciated by cytocentrifuge and cell block as
compared to routine centrifuge.
OBJECTIVE
To compare conventional smear, cytospin smear and cell block technique in
analyzing cytology of body fluids.
MATERIALS AND METHODS
A prospective hospital based study will be carried out on patients fulfilling the
inclusion criteria, referred to the Department of Pathology in BLDEU’S ShriB.M.Patil
Medical College, Hospital and Research centre, Vijayapur.
Study period:1st December 2014 to 30th June 2016RESULTS
Inthisstudyminimal amount of background blood and proteinaceous material in
65% of cytospin smears 5% of conventional smears and 46.2% of cell blocks. Abundant
cellularity was seen in 47.5% of cytospin smears 23.8% of conventional smears and 5%
of cell blocks. Minimal cell degeneration was noted in 82.5% of cytospin smears 53.8%
of conventional smears and 45% of cell blocks. Even distribution of cells was seen in
76.2% of cytospin smears 8.8% of conventional smears and 17.5% of cell blocks.
CONCLUSION
Cytospin smears showed clear background, high cellularity, better nuclear
features and even distribution of cells.Hence cytospin smears are better when compared
to centrifuge and cell block in analysing body fluids.
