Comparative Study Of Imprint And Scrape Cytology In The Diagnosis Of Tumour And Tumour Like Lesions Of Ovary

Abstract

Introduction: Tumour and tumour like lesions of ovary are the most frequently encountered ovarian lesions in females. In the present day, utility of preoperative and intraoperative diagnostic cytology is increasing. Various cytological methods such as Imprint and Scrape cytology can be used for quick microscopic analysis of a pathological lesion and helps to differentiate between neoplastic and non neoplastic conditions. Objectives: To evaluate utility of imprint and scrape cytology in tumour and tumour like lesions of ovary. Materials and Methods: A prospective study was done on surgically resected specimens of ovary from December, 2017 to June, 2019. For imprint smears, slides were gently touched on freshly cut surface of specimen. For scrape smears, cut surface of ovary was scraped with one end of the slide and smear was prepared on the other slide. These smears were immediately fixed in 95% ethanol and stained with Haematoxylin & Eosin stain and PAP stain and cytomorphological study of smears was done which was further correlated with histopathology diagnosis. Results: Total 110 cases were studied, 68 (61.8%) cases were epithelial tumours, 18(16.4%) cases were germ cell tumours, 12(10.9%) cases were diagnosed as sex cord stromal tumours, 1 case each of lymphoma and metastatic carcinoma (0.9% each) and 10 (9.1%) cases were tumour like lesions of ovary. Cytohistological discordance was found in 17 cases when compared to histopathological diagnosis. Sensitivity, Specificity, Positive predictive value and Accuracy of imprint and scrape cytology was 88%, 98.7%, 91.6% and 91%respectively. Comparison between imprint and scrape smears was done for cytomorphological features such as cellularity, architectural pattern, nuclear features, cytoplasmic staining and background. Difference between imprint and scrape for cytomorphological features was statistically significant at 5% level of significance. Conclusion: Scrape cytology provides an efficient means of investigation and is having a high degree of sensitivity, specificity and accuracy; hence it can be applied for cytomorphological analysis of tumour and tumour like lesions of ovary as a rapid diagnostic modality in adjunct to frozen section.