Antibiogram and Isolation of S. aureus from the Urinary Tract Infections: Comparison of Meca Gene Detection and Phenotypic Methods for Detection of Methicillin-Resistant S. aureus

Abstract

Introduction: Urinary tract infections (UTIs) are one of the most common infectious diseases, and nearly 10% of people will experience a UTI during their lifetime. S. aureus is one of the most widely spread human pathogens. Objective: To detect Methicillin-resistant Staphylococcus aureus(MRSA) among S. aureus causing UTI. Objectives: To know the antibiotic sensitivity pattern of the isolates and comparison of mecA gene detection and phenotypic methods for the detection of MRSA. Methods: This study conducted over 2 years from January 2017 to December 2018. The isolates were identified by standard protocol. All isolates were tested for antimicrobial susceptibility. MRSA was identified by Cefoxitin and Oxacillin disk diffusion method. mecA gene was detected by PCR. Results: UTI were reported more among females in the age group of 21 to 40 with a rate of 42%. Among the male patients, UTI was reported more in elderly patients with 50% of cases occurring between the age group of 40-60 years. Linezolid was found to be the most effective drug overall against S.aureus. The highest percentages of resistance were found for penicillin and pefloxacin. Cefoxitin and Oxacillin detected 19 and 15 isolates as MRSA respectively, the PCR detected mec A genes in 20 isolates. Conclusion: UTIs were more among young females patients and elderly male patients. PCR was the best method for the detection of MRSA. Cefoxitin disc is the best alternatives for PCR for the identification of MRSA. Antibiotic sensitivity revealed MRSA were resistant to many antibiotics but were sensitive to tetracycline, gentamicin, vancomycin and linezolid.